acs.est.9b02339.pdf (2.02 MB)
Sustained dechlorination of vinyl chloride to ethene in dehalococcoides-enriched cultures grown without addition of exogenous vitamins and at low pH
journal contribution
posted on 2020-05-27, 14:04 authored by Luz A Puentes Jácome, Po-Hsiang Wang, Olivia Molenda, Yi Xuan Jine-Jine Li, Ahsan IslamAhsan Islam, Elizabeth A Edwards© 2019 American Chemical Society. Trichloroethene (TCE) bioremediation has been demonstrated at field sites using microbial cultures harboring TCE-respiring Dehalococcoides whose growth is cobalamin (vitamin B12)-dependent. Bioaugmentation cultures grown ex situ with ample exogenous vitamins and at neutral pH may become vitamin-limited or inhibited by acidic pH once injected into field sites, resulting in incomplete TCE dechlorination and accumulation of vinyl chloride (VC). Here, we report growth of the Dehalococcoides-containing bioaugmentation culture KB-1 in a TCE-amended mineral medium devoid of vitamins and in a VC-amended mineral medium at low pH (6.0 and 5.5). In these cultures, Acetobacterium, which can synthesize 5,6-dimethylbenzimidazole (DMB), the lower ligand of cobalamin, and Sporomusa are dominant acetogens. At neutral pH, Acetobacterium supports complete TCE dechlorination by Dehalococcoides at millimolar levels with a substantial increase in cobalamin (∼20-fold). Sustained dechlorination of VC to ethene was achieved at pH as low as 5.5. Below pH 5.0, dechlorination was not stimulated by DMB supplementation but was restored by raising pH to neutral. Cell-extract assays revealed that vinyl chloride reductase activity declines significantly below pH 6.0 and is undetectable below pH 5.0. This study highlights the importance of cobamide-producing populations and pH in microbial dechlorinating communities for successful bioremediation at field sites.
Funding
Genome Canada, the Ontario Genomics Institute [2009-OGI-ABC-1405]
NSERC CREATE RENEW program [180804567],
Ontario Research Fund INTEGRATE project [ORF-RE05-WR-01]
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Environmental Science & TechnologyVolume
53Issue
19Pages
11364 - 11374Citation
Jacome, L.A.P. ... et al., 2020. Sustained Dechlorination of Vinyl Chloride to Ethene in Dehalococcoides-Enriched Cultures Grown without Addition of Exogenous Vitamins and at Low pH. Environmental Science & Technology, 53(19), pp. 11364 - 11374.Publisher
American Chemical Society (ACS)Version
- VoR (Version of Record)
Rights holder
© American Chemical SocietyPublisher statement
This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Jacome, L.A.P. ... et al., 2020. Sustained Dechlorination of Vinyl Chloride to Ethene in Dehalococcoides-Enriched Cultures Grown without Addition of Exogenous Vitamins and at Low pH. Environmental Science & Technology, 53(19), pp. 11364 - 11374 and may be found at https://doi.org/10.1021/acs.est.9b02339Acceptance date
2019-08-23Publication date
2019-08-23Copyright date
2019ISSN
0013-936XeISSN
1520-5851Publisher version
Language
- en
Location
United StatesDepositor
Dr Ahsan Islam Deposit date: 22 May 2020Usage metrics
Categories
No categories selectedKeywords
Science & TechnologyTechnologyLife Sciences & BiomedicineEngineering, EnvironmentalEnvironmental SciencesEngineeringEnvironmental Sciences & EcologySP-NOVREDUCTIVE DECHLORINATIONSPOROMUSA-OVATACHLORINATED ETHENESLOWER LIGANDTETRACHLOROETHENEBIOSYNTHESISCORRINOIDSCOBAMIDEBACTERIABiodegradation, EnvironmentalChloroflexiEthylenesHydrogen-Ion ConcentrationTrichloroethyleneVinyl ChlorideVitamins
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC