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Synergy between myogenic and non-myogenic cells in a 3D tissue-engineered craniofacial skeletal muscle construct

journal contribution
posted on 11.09.2014 by Mariea A Brady, Mark Lewis, Vivek Mudera
In vitro skeletal muscle engineering involves the culture of isolated primary myogenic cells in an environment conducive to the formation of a three-dimensional (3D) tissue construct capable of generating force. Isolated human myogenic cells have been used to study cell-cell interactions, permitting identification of functions intrinsic to skeletal muscle in two dimensions (2D). However, the independent contribution of human myogenic and non-myogenic cell types that comprise skeletal muscle to myogenic cell differentiation, force generation and matrix remodelling has yet to be established in 3D. The objective of this study was to use isolated human myogenic and nonmyogenic muscle-derived cells (MDC) seeded in 3D collagen constructs to engineer a biomimetic craniofacial skeletal construct. The aim was to purify the two subpopulations of myogenic and non-myogenic cells from human masseter muscle and quantitate myogenic cell differentiation, force generation and matrix remodelling of the 3D collagen construct. The results showed that both the heterogeneous mixture of cells and the purified myogenic cell population expressed myogenin, indicative of myogenic cell differentiation. Further, there was a synergistic effect as the heterogeneous co-culture of myogenic and non-myogenic cells generated the highest peak force and expressed the most MMP-2 mRNA compared to isolated individual cell populations. Copyright © 2008 John Wiley & Sons, Ltd.

Funding

Royal National Orthopaedic Hospital NHS Trust R&D funding

History

School

  • Sport, Exercise and Health Sciences

Published in

Journal of Tissue Engineering and Regenerative Medicine

Volume

2

Issue

7

Pages

408 - 417

Citation

BRADY, M.A., LEWIS, M.P. and MUDERA, V., 2008. Synergy between myogenic and non-myogenic cells in a 3D tissue-engineered craniofacial skeletal muscle construct. Journal of Tissue Engineering and Regenerative Medicine, 2 (7), pp. 408 - 417.

Publisher

© John Wiley & Sons, Ltd.

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2008

Notes

Closed access

ISSN

1932-6254

eISSN

1932-7005

Language

en

Exports