Clifford et al. 2017 Tregs.pdf (2.39 MB)
T-regulatory cells exhibit a biphasic response to prolonged endurance exercise in humans
journal contribution
posted on 2019-10-21, 09:22 authored by Tom CliffordTom Clifford, Matthew J Wood, Philip Stocks, Glyn Howatson, Emma J Stevenson, Catharien MU HilkensPurpose: T-regulatory cells (Tregs) are a sub-population of lymphocytes that act to suppress aberrant immune responses. We investigated changes in the numbers of naïve and terminally differentiated Tregs in the peripheral blood to establish their role in the immuno-suppressive response to prolonged exercise. Methods: Blood was drawn from seventeen experienced runners (age 40 ± 12 years; height 1.75 ± 0.08 m; mass 71.4 ± 10.8 kg) before, ~1 h after (POST-1h), and on the day following the marathon (POST-1d). Tregs (CD3+CD4+Foxp3+CD25++CD127−) were analysed in peripheral blood mononuclear cells using flow cytometry. The markers CD45RA and HLA-DR were included to define naïve and terminally differentiated Tregs, respectively. Results: The absolute number of Tregs decreased (27%) POST-1h marathon (P < 0.001) but increased (21%) at POST-1d (P < 0.01). Naïve CD45RA+ Tregs fell by 39% POST-1h (P < 0.01) but were unaffected POST-1d (P > 0.05). In contrast, an increased number of Tregs expressing HLA-DR was observed at POST-1d (P < 0.01). Interleukin (IL)-1β, IL-6, IL-8 and IL-10 levels in the serum all increased POST-1h (P > 0.05) but returned to pre-exercise levels POST-1d. The suppressive cytokine, transforming growth factor-beta, was unaffected by the marathon (P > 0.05). Conclusions: These results suggest that Tregs do not play a major role in immune suppression in the early hours of recovery from a marathon. However, terminally differentiated HLA-DR+ Tregs are mobilized the following day, which could represent a compensatory attempt by the host to restore immune homeostasis and limit excessive cell damage.
History
School
- Sport, Exercise and Health Sciences
Published in
European Journal of Applied PhysiologyVolume
117Issue
8Pages
1727 - 1737Publisher
SpringerVersion
- VoR (Version of Record)
Rights holder
© The AuthorsAcceptance date
2017-06-17Publication date
2017-06-23Copyright date
2017ISSN
1439-6319eISSN
1439-6327Publisher version
Language
- en
Depositor
Dr Tom Clifford. Deposit date: 18 October 2019Usage metrics
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