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The use of shift reagents in ion mobility-mass spectrometry: studies on the complexation of an active pharmaceutical ingredient with polyethylene glycol excipients

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journal contribution
posted on 25.03.2009 by Mark D. Howdle, Christine Eckers, Alice M.-F. Laures, Colin Creaser
Gas-phase ion mobility studies of mixtures containing polyethylene glycols (PEG) and an active pharmaceutical ingredient (API), Lamivudine, have been carried out using electrospray ionization-ion mobility spectrometry-quadrupole-time-of-flight mass spectrometry (ESI-IMS-Q-TOF). In addition to protonated and cationised PEG oligomers, a series of high molecular weight ions were observed and identified as non-covalent complexes formed between Lamivudine and PEG oligomers. The non-covalent complex ions were dissociated using collision induced dissociation (CID) after separation in the ion mobility drift tube to recover the protonated Lamivudine free from interfering matrix ions and with a drift time associated with the precursor complex. The potential of PEG excipients to act as ‘shift reagents’, which enhance selectivity by moving the mass/mobility locus to an area of the spectrum away from interferences, is demonstrated for the analysis of Lamivudine in a Combivir formulation containing PEG and Lamivudine.

History

School

  • Science

Department

  • Chemistry

Citation

HOWDLE, M. ... et al, 2009. The use of shift reagents in ion mobility-mass spectrometry: studies on the complexation of an active pharmaceutical ingredient with polyethylene glycol excipients. Journal of the American Society for Mass Spectrometry, 20 (1), pp.1-9

Publisher

Elsevier / © American Society for Mass Spectrometry

Version

AM (Accepted Manuscript)

Publication date

2009

Notes

This article was published in the journal, Journal of the American Society for Mass Spectrometry [© American Society for Mass Spectrometry] and the definitive version is available at: http://dx.doi.org/10.1016/j.jasms.2008.10.002

ISBN

1044-0305

Language

en

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