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Urinary extracellular vesicles: Potential biomarkers of renal function in diabetic patients

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posted on 25.01.2017 by Agnieszka Kaminska, Mark Platt, Joanna Kasprzyk, Beata Kusnierz-Cabala, Agnieszka Gala-Bladzinska, Olga Woznicka, Benedykt R. Jany, Franciszek Krok, Wojciech Piekoszewski, Marek Kuzniewski, Ewa L. Stepien
The aim of this study was to check the relationship between the density of urinary EVs, their size distribution, and the progress of early renal damage in type 2 diabetic patients (DMt2). Patients were enrolled to this study, and glycated hemoglobin (HbA1c) below 7% was a threshold for properly controlled diabetic patients (CD) and poorly controlled diabetic patients (UD). Patients were further divided into two groups: diabetic patients without renal failure (NRF) and with renal failure (RF) according to the Glomerular Filtration Rate. Density and diameter of EVs were determined by Tunable Resistive Pulse Sensing. Additionally, EVs were visualized by means of Transmission and Environmental Scanning Electron Microscopy. Nano-liquid chromatography coupled offline with mass spectrometry (MALDI-TOF-MS/MS) was applied for proteomic analysis. RF had reduced density of EVs compared to NRF.The size distribution study showed that CD had larger EVs (mode) than UD (115 versus 109 nm; 𝑝 < 0.05); nevertheless themean EVs diameter was smaller in controls than in the CD group (123 versus 134 nm; 𝑝 < 0.05). It was demonstrated that EVs are abundant in urine. Albumin, uromodulin, and number of unique proteins related to cell stress and secretion were detected in the EVs fraction. Density and size of urinary EVs reflect deteriorated renal function and can be considered as potential renal damage biomarkers.

History

School

  • Science

Department

  • Chemistry

Published in

Journal of Diabetes Research

Volume

2016

Pages

1 - 1 (12)

Citation

KAMINSKA, A. ...et al., 2017. Urinary extracellular vesicles: Potential biomarkers of renal function in diabetic patients. Journal of Diabetes Research, 2016: 5741518.

Publisher

© The Authors. Published by the Hindawi Publishing Corporation

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Acceptance date

13/11/2016

Publication date

2016-12-25

Notes

This is an Open Access Article. It is published by Hindawi Publishing Corporation under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

ISSN

2314-6753

Language

en

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