Chem Commun 2015 51, 1579-1582.pdf (2.18 MB)
Virtues and limitations of Pittsburgh green for ozone detection
journal contribution
posted on 2015-02-25, 11:12 authored by Carlos Castello Beltran, Elliott A. Palmer, Benjamin BuckleyBenjamin Buckley, Felipe IzaFelipe IzaA recently proposed 2′,7′-dichlorofluorescein (DCF)-derived fluorescent probe for the detection of ozone shows good selectivity against a number of reactive oxygen species and good pH stability for biological and environmental applications. It is found, however, that over oxidation of the fluorescent product (Pittsburgh green) can occur. This could render quantitative measurements inaccurate due to a reduction in fluorescence and overlapping fluorescence signals from over oxidation by-products and it requires careful experimental design. Although difficult to assess by fluorescence measurements, the over oxidation can be conveniently monitored by 1H NMR spectroscopy.
Funding
We thank Loughborough University for a PhD studentship (to C.C.-B.), Research Councils UK for a RCUK Fellowship (to B.R.B.) and the UK Engineering and Physical Science Research Council (F.I.)
History
School
- Science
Department
- Chemistry
Published in
Chemical CommunicationsVolume
51Issue
9Pages
1579 - 1582Citation
CASTELLO BELTRAN, C. ... et al., 2015. Virtues and limitations of Pittsburgh green for ozone detection. Chemical Communications, 51 (9), pp. 1579 - 1582.Publisher
Royal Society of ChemistryVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/Publication date
2015Notes
This is an Open Access Article. It is published by Royal Society of Chemistry under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/ISSN
1359-7345eISSN
1364-548XPublisher version
Language
- en