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Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale

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posted on 15.09.2015, 10:07 by Xiaowei Zhang, Matthieu Stettler, Dario De Sanctis, Marco Perrone, Nicola Parolini, Marco DiscacciatiMarco Discacciati, Maria De Jesus, David Hacker, Alfio Quarteroni, Florian M. Wurm
Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel mammalian cell culture bioreactor systems for the rapid production of biologicals that require mammalian protein processing. Recently, orbitally shaken bioreactors at scales from 50 mL to 1,000 L have been explored for the cultivation of mammalian cells and are considered to be attractive alternatives to conventional stirred-tank bioreactors because of increased flexibility and reduced costs. Adequate oxygen transfer capacity was maintained during the scale-up, and strategies to increase further oxygen transfer rates (OTR) were explored, while maintaining favorable mixing parameters and low-stress conditions for sensitive lipid membrane-enclosed cells. Investigations from process development to the engineering properties of shaken bioreactors are underway, but the feasibility of establishing a robust, standardized, and transferable technical platform for mammalian cell culture based on orbital shaking and disposable materials has been established with further optimizations and studies ongoing.

History

School

  • Science

Department

  • Mathematical Sciences

Volume

115

Pages

33 - 53

Citation

ZHANG, X. ... et al, 2009. Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale. IN: Eibl, R. and Eibl, D. (eds). Disposable Reactors. Berlin: Springer Berlin Heidelberg, pp.33-53.

Publisher

© Springer-Verlag Berlin Heidelberg

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2009

Notes

This paper is closed access.

ISBN

9783642018725;9783642018718

ISSN

0724-6145

Book series

Advances in Biochemical Engineering / Biotechnology;115

Language

en