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Creating aptamers and their use in resisitive pulse sensors

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conference contribution
posted on 13.03.2018 by Mark Platt
Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse small channels. Here we present our recent advancement of the technique identifying key experimental designs for potential POC assays. The first assay utilized superparamagnetic beads, if the surfaces of the beads are modified with an aptamer, the frequency of beads (translocations/minute) through the pore can be related to the concentration of specific proteins in the solution. Herein, we have demonstrated the successful use of TRPS to observe the binding of two proteins, to their specific aptamers simultaneously. We then adapt the measurement strategy and demonstrate that the translocation times of particles can be used to infer the zeta potential to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridisation time, we observe a clear difference in zeta potential using both mean values, and population distributions as a function of the DNA structure. Finally we present the first comparison between assays that use resistive pulses or rectification ratios on a tunable pore platform.

History

School

  • Science

Department

  • Chemistry

Published in

Biosensors 2016 Procedia Technology

Volume

27

Pages

12 - 13

Citation

PLATT, M., 2017. Creating aptamers and their use in resisitive pulse sensors. Procedia Technology, 27, pp.12-13.

Publisher

Elsevier © The Author

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2017

Notes

This is an Open Access Article. It is published by Elsevier under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (CC BY-NC-ND). Full details of this licence are available at: http://creativecommons.org/licenses/by-nc-nd/4.0/. This paper was also presented at Biosensors 2016, Gothenburg, Sweden, 25-27 May 2016.

ISSN

2212-0173

Language

en

Location

Gothenburg, Sweden

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