Supplementary information files for "Phenotypic plasticity of a Baetid mayfly larvae (Baetis rhodani) at sites with high levels of deposited fine sediment"

Supplementary files for article "Phenotypic plasticity of a Baetid mayfly larvae (Baetis rhodani) at sites with high levels of deposited fine sediment"

1. Excess fine sediment (particles <2 mm) delivery and deposition in freshwater systems is a significant factor in structuring aquatic communities and populations.
2. Invertebrate gill surfaces can become covered with fine sediment, potentially compromising osmoregulatory function.
3. Ionocytes are specialised structures for osmoregulation found on the tracheal gills of mayflies. The number of cells has been shown to change in order to maintain osmoregulatory demands under environmentally variable conditions.
4. To investigate whether ionocytes vary in response to fine sediment pressure, individuals of Baetis rhodani were collected from two high and low fine sediment cover sites, respectively, in the UK. Tracheal gills were subsequently examined for the number of ionocytes present on the upper and lower gill surfaces, standardised by gill size.
5. Results indicated that the number of ionocytes was significantly higher for mayflies collected from areas with high fine sediment cover. High fine sediment sites were also characterised by lower altitude and dissolved oxygen concentrations. Mayfly gills collected from high sediment cover sites were also significantly larger than those collected from low sediment sites.
6. The results illustrate the potential for mayfly larvae to demonstrate phenotypic plasticity to the pressures associated with fine sediment but that these responses are likely dependent on the composition of fine sediment deposits (organic or mineral) and associated oxygen concentrations.

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