Imaging of two reporter genes in the same mouse
figure
posted on 2011-12-30, 14:02 authored by Frank Berger, Sanjiv Sam GambhirCopyright information:
Taken from "Breast imaging technology: Recent advances in imaging endogenous or transferred gene expression utilizing radionuclide technologies in living subjects - applications to breast cancer"
Breast Cancer Research 2000;3(1):28-35.
Published online 11 Dec 2000
PMCID:PMC139436.
Copyright © 2000 BioMed Central Ltd
A nude mouse carrying two tumors (left, dopamine 2 receptor [D2R]; right, herpes simplex virus type 1 thymidine kinase []) was imaged in a microPET system with tail-vein injection of ~ 250 μCi [F]-fluoroethylspiperone (FESP) and, 24 hours later, with ~ 250 μCi 8-[F]-fluoropenciclovir (FPCV). Imaging began 1 hour after injection of each tracer. These results show the ability to image both the D2R and the reporter genes in the same living mouse with microPET. The scale represents the accumulation of FESP and FPCV measured as percent injected dose per gram of tissue (%ID/g).