File(s) not publicly available

Reason: This item is currently closed access.

A compact long-wavelength fluorescence detection system and its application to flow injection immunoassay

journal contribution
posted on 08.08.2016 by Sijung Hu, Martin T. French, Derek A. Palmer, Mark Evans, Sue M. Zhou, G. Hussain Sarpara, James N. Miller
A compact and robust long-wavelength fluorescence detection system (LWFDS) has been constructed using a pulsed diode laser as the light source and a photodiode detector. The detection limits for naphthofluorescein (NF) and the cyanine dyes Cy5 and Cy5.5 at pH 8.8, determined using the LWFDS, were 1.0 × 10-10, 2.3 × 10-12 and 6.2 × 10-11M, respectively. These values compared favourably with data from a commercial research grade fluorescence spectrometer. The LWFDS instrument was used as the detector for a competitive heterogeneous flow injection immunoassay for α-interferon with Cy5 as the label. A calibration graph usable over the range 0.5-10 μg ml-1 α-interferon was obtained. The instrument demonstrated its potential for rapid ( <200s per sample) multi-analyte assays and for field monitoring.

Funding

S.J.H. acknowledges financial support from Loughborough University and from Kalibrant Limited. We also thank Glaxo-Wellcome for gifts of α-interferon and its antibodies.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Analytica Chimica Acta

Volume

454

Issue

1

Pages

31 - 35

Citation

HU, S. ... et al., 2002. A compact long-wavelength fluorescence detection system and its application to flow injection immunoassay. Analytica Chimica Acta, 454 (1), pp.31-35.

Publisher

© Elsevier

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2002

Notes

Closed access.

ISSN

0003-2670

Language

en

Exports

Logo branding

Exports