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A miniaturised integrated biophotonic point-of-care genotyping system

journal contribution
posted on 09.07.2020 by John M Girkin, Mazher Mohammed, Elizabeth M Ellis
This paper reports the development of a novel genotyping device specifically designed for point-of-care applications. As the results of the human genome project are applied to clinical practice there is an increasing requirement for simple to operate high-speed, potentially low-cost genotyping devices for use in the clinic. The aim of such devices is not to specifically detect a full gene sequence but to monitor the presence of specific Single Nucleotide Polymorphisms (SNPs). The instrument is designed to fulfil this specific clinical requirement. Using a FRET-based assay the instrument completes a full PCR process and then performs a melting point test to determine the exact SNPs present in the sample. Results are presented in which the instrument produces results within 18 min based upon saliva samples provided by the patient. The paper also reports successful results both with purified DNA samples and saliva-based samples which were taken from subjects after experiments deliberately aimed at confusing the instrument.

Funding

Engineering and Physical Sciences Research Council (EPSRC)

History

School

  • Design

Published in

Faraday Discussions

Volume

149

Pages

115 - 123

Publisher

Royal Society of Chemistry (RSC)

Version

VoR (Version of Record)

Rights holder

© The Royal Society of Chemistry

Publisher statement

This paper was accepted for publication in the journal Faraday Discussions and the definitive published version is available at https://doi.org/10.1039/c005271j.

Acceptance date

19/07/2010

Publication date

2010-09-24

Copyright date

2011

ISSN

1359-6640

eISSN

1364-5498

Language

en

Depositor

Dr Mazher Mohammed. Deposit date: 8 July 2020

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