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A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation

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journal contribution
posted on 03.08.2016, 08:42 by G. Hussain Sarpara, Sijung Hu, Derek A. Palmer, Martin T. French, Mark Evans, James N. Miller
Naphthofluorescein diphosphate has been synthesised from the parent dye, and shown to be an attractive longwavelength alternative to other fluorigenic substrates for the determination of alkaline phosphatase. Its application to the determination of theophylline, an inhibitor of this enzyme, has been demonstrated. The optimum excitation wavelength of the hydrolysis product naphthofluorescein has been found to depend on the presence of additives such as cyclodextrins and (3-[3-cholamidopropyl]-dimethylamino)-1-propane sulfonate (CHAPS): such effects can be used to raise the excitation wavelength to match the output of a 635 nm diode laser in a simple and sensitive fluorescence detector.

Funding

G.H.S. thanks the Government of Pakistan for financial support.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Analytical Communications

Pages

. 19 - 20

Citation

SARPARA, G. ... et al., 1999. A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation. Analytical Communications, 36, pp.19-20.

Publisher

© Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

1999

ISSN

1359-7337

eISSN

1364-5536

Language

en

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