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Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies

journal contribution
posted on 26.02.2015 by Alexandra Stolzing, E. Jones, D. McGonagle, Andrew Scutt
Human mesenchymal stem cells (hMSC) represent a promising cell-based therapy for a number of degenerative conditions. Understanding the effect of aging on hMSCs is crucial for autologous therapy development in older subject whom degenerative diseases typically afflict. Previous investigations into the effects of aging on hMSC have proved contradictory due to the relative narrow age ranges of subjects assessed and the exclusive reliance of in vitro assays. This study seeks to address this controversy by using a wider range of donor ages and by measuring indices of cellular aging as well as hMSC numbers ex vivo and proliferation rates. CFU-f analysis and flow cytometry analysis using a CD45 low/D7fib +ve/LNGF +ve gating strategy were employed. In addition a variety of markers of cellular aging, oxidative damage and senescence measured. A reduction in CFU-f and CD45 low/D7fib +ve/LNGF +ve cell numbers were noted in adulthood relative to childhood. Indices of aging including oxidative damage, ROS levels and p21 and p53 all increased suggesting a loss of MSC fitness with age. These data suggest that hMSC numbers obtained by marrow aspiration decline with age. Furthermore, there is an age-related decline in overall BM MSC "fitness" which might lead to problems when using autologous aged MSC for cell-based therapies. © 2007 Elsevier Ireland Ltd. All rights reserved.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Mechanisms of Ageing and Development

Volume

129

Issue

3

Pages

163 - 173

Citation

STOLZING, A. ... et al, 2008. Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies. Mechanisms of Ageing and Development, 129 (3), pp. 163 - 173.

Publisher

© Elsevier Ireland Ltd

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2008

Notes

This article is closed access.

ISSN

0047-6374

Language

en

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