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An investigation into the preservation of microbial cell banks for alpha-amylase production during 5L fed-batch Bacillus licheniformis fermentations

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journal contribution
posted on 08.11.2010 by Nichola H. Hancocks, Colin R. Thomas, Stuart M. Stocks, Christopher Hewitt
Fluorescent staining techniques were used for a systematic examination of methods used to cryopreserve microbial cell banks. The aim of cryopreservation here is to ensure subsequent reproducible fermentation performance rather than just post thaw viability. Bacillus licheniformis cell physiology post-thaw is dependent on the cryopreservant (either Tween 80, glycerol or dimethyl sulphoxide) and whilst this had a profound effect on the length of the lag phase, during subsequent 5 l fed-batch fermentations, it had little effect on maximum specific growth rate, final biomass concentration or a-amylase activity. Tween 80 not only protected the cells during freezing but also helped them recover post-thaw resulting in shorter process times.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Citation

HANCOCKS, N.H. ... et al, 2010. An investigation into the preservation of microbial cell banks for alpha-amylase production during 5L fed-batch Bacillus licheniformis fermentations. Biotechnology Letters, 32 (10), pp. 1405-1412.

Publisher

© Springer

Version

AM (Accepted Manuscript)

Publication date

2010

Notes

The final publication is available at www.springerlink.com

ISSN

0141-5492

Language

en

Exports