Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator
journal contributionposted on 09.05.2018 by Samantha Swarbrick, Igor Efimov, Victor P. Ostanin, David Klenerman, Alexandra Stolzing, Sourav Ghosh
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The paper describes a sensor for single-stranded DNA (ssDNA) biomarker based on anharmonic acoustic signals arising during hybridization with complementary thiolated ssDNA functionalised on the gold electrode of an electrochemical quartz crystal resonator. The steps of sensor preparation and hybridization are carried out in an electrochemical microfluidic flowcell. While the electrochemical impedance spectroscopy does not allow a definitive interpretation, the changes in resonance frequency and third Fourier harmonic current of the resonator on actuation at the fundamental mode indicate formation of a flexibly bound layer. The functionalization and hybridization steps monitored by the anharmonic detection technique (ADT) are described with a simple model based on Duffing nonlinear equation.
The authors sincerely acknowledge the funding support from the EU projects RAPP-ID (FP7-JTI 115153) and Norosensor (FP7-NMP 604244), EPSRC Bridging the Gap in Antimicrobial Resistancegrant (EP/M027341/1) and PhD studentship support for SS from EPSRC Doctoral Training Centre (EP/F500491/1).
- Mechanical, Electrical and Manufacturing Engineering