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Automated adherent human cell culture (mesenchymal stem cells)

journal contribution
posted on 21.08.2017 by Rob Thomas, Elizabeth Ratcliffe
Human cell culture processes developed at research laboratory scale need to be translated to large-scale production processes to achieve commercial application to a large market. To allow this transition of scale with consistent process performance and control of costs, it will be necessary to reduce manual processing and increase automation. There are a number of commercially available platforms that will reduce manual process intervention and improve process control for different culture formats. However, in many human cell-based applications, there is currently a need to remain close to the development format, usually adherent culture on cell culture plastic or matrix-coated wells or flasks due to deterioration of cell quality in other environments, such as suspension. This chapter presents an example method for adherent automated human stem cell culture using a specific automated flask handling platform, the CompacT SelecT.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Methods in Molecular Biology

Volume

806

Pages

393 - 406

Citation

THOMAS, R. and RATCLIFFE, E., 2012. Automated adherent human cell culture (mesenchymal stem cells). IN: Mitry, R. and Hughes, R. (eds.). Human Cell Culture Protocols. (Methods in Molecular Biology ; vol. 806.) London: Springer, pp.393-406.

Publisher

© Springer

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2012

ISBN

9781617793660;9781617793677

ISSN

1064-3745

eISSN

1940-6029

Book series

Methods in Molecular Biology;806

Language

en

Exports