File(s) under permanent embargo

Reason: This item is currently closed access.

Automated adherent human cell culture (mesenchymal stem cells)

journal contribution
posted on 21.08.2017, 10:08 by Rob Thomas, Elizabeth Ratcliffe
Human cell culture processes developed at research laboratory scale need to be translated to large-scale production processes to achieve commercial application to a large market. To allow this transition of scale with consistent process performance and control of costs, it will be necessary to reduce manual processing and increase automation. There are a number of commercially available platforms that will reduce manual process intervention and improve process control for different culture formats. However, in many human cell-based applications, there is currently a need to remain close to the development format, usually adherent culture on cell culture plastic or matrix-coated wells or flasks due to deterioration of cell quality in other environments, such as suspension. This chapter presents an example method for adherent automated human stem cell culture using a specific automated flask handling platform, the CompacT SelecT.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Methods in Molecular Biology

Volume

806

Pages

393 - 406

Citation

THOMAS, R. and RATCLIFFE, E., 2012. Automated adherent human cell culture (mesenchymal stem cells). IN: Mitry, R. and Hughes, R. (eds.). Human Cell Culture Protocols. (Methods in Molecular Biology ; vol. 806.) London: Springer, pp.393-406.

Publisher

© Springer

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2012

ISBN

9781617793660;9781617793677

ISSN

1064-3745

eISSN

1940-6029

Book series

Methods in Molecular Biology;806

Language

en

Exports