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Automated, scalable culture of human embryonic stem cells in feeder free conditions

journal contribution
posted on 17.02.2009 by Rob Thomas, David Anderson, Amit Chandra, Nigel M. Smith, Lorraine E. Young, David Williams, Chris Denning
Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Citation

THOMAS, R.J. ... et al, 2009. Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnology and Bioengineering, 102 (6), pp. 1636-1644

Publisher

© Wiley Periodicals

Version

NA (Not Applicable or Unknown)

Publication date

2009

Notes

This article is Restricted Access. It was published in the journal, Biotechnology and Bioengineering [© Wiley] and is available at: http://www3.interscience.wiley.com/journal/117933915/grouphome/home.html

ISSN

0006-3592

Language

en

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