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Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila

journal contribution
posted on 07.12.2015 by Marco Lucas, Carla Amaral, Ana Sampaio, Jose A. Peres, Albino A. Dias
This work looks for a better understanding of the biodegradation of xenobiotic azo dyes mediated by yeasts. During a screening program of phenolic acid assimilating capacities it was found that a non-conventional ascomycetous yeast isolate, identified as Candida oleophila, efficiently decolorizes agar plates supplemented with the commercial textile diazo dye Reactive Black 5. Aerobic batch cultures of C. oleophila could completely decolorize up to 200 mg dye l−1, an ability not yet reported for this yeast species. Moreover, this performance has been achieved in just 24 h of incubation at 26 ◦C in the presence of as little as 5 g glucose l−1 and without visible signs of dye adsorption to yeast cells. It was found that decolorization occurs during the exponential growth phase and neither laccase nor manganese-dependent peroxidase activities were detected in the culture medium. As far as the decolorization mechanism is concerned, our results indirectly suggest the involvement of an azoreductase-like activity in azo bonds cleavage.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Enzyme and Microbial Technology

Volume

39

Issue

1

Pages

51 - 55

Citation

LUCAS, M.S. ... et al, 2006. Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme and Microbial Technology, 39 (1), pp.51-55

Publisher

© Elsevier Inc

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2006

Notes

This paper is Closed Access.

ISSN

0141-0229

eISSN

1879-0909

Language

en

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