26835.pdf (226.08 kB)

Challenges in manufacturing an amniotic membrane alternative for corneal regeneration [abstract]

Download (226.08 kB)
journal contribution
posted on 05.10.2017, 16:26 by Sammy Wilson, Andrew Hopkinson
The current worldwide cornea shortages have led to the development of feasible, long-term substitutes to cadaveric donor tissue. Amniotic membrane is a clinically successful material used to reconstruct damaged corneal surfaces. However, its reliance upon the host tissue, availability, donor variation, infection risks and the fact that it is currently unsuitable for complete corneal regeneration have warranted the need for alternative solutions. Alternatives in current clinical or pre-clinical development include keratoprostheses, tissue engineered constructs, xenografts and the use of acellular matrices. With respect to corneal regeneration, there are many challenges, not least that the corneal structure is unique and difficult to replicate. When manufacturing corneal tissues, the choice of material is vital as the list of requirements is extensive. They must be biocompatible, (preferably) optically transparent, flexible, and strong, as to withstand manipulation in culture, potential suturing, irrigation and handling during surgery. Manufacturing process need to be simple and consistent, preferably at high speed and low cost. Two fundamental objectives of corneal regeneration are the maintenance of healthy cell phenotypes and the replication of the native tissue architecture. If both factors are not satisfied, the result is often regenerated tissue mimicking that of scarred native tissue.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Acta Ophthalmologica

Volume

91

Pages

0 - 0

Citation

WILSON, S. and HOPKINSON, A., 2013. Challenges in manufacturing an amniotic membrane alternative for corneal regeneration [abstract]. Acta Ophthalmologica, 91, S252, DOI: 10.1111/j.1755-3768.2013.1624.x.

Publisher

John Wiley & Sons (© Acta Ophthalmologica Scandinavica Foundation)

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2013

Notes

This is the peer reviewed version of the following article: WILSON, S. and HOPKINSON, A., 2013. Challenges in manufacturing an amniotic membrane alternative for corneal regeneration [abstract]. Acta Ophthalmologica, 91, S252, which has been published in final form at: https://doi.org/10.1111/j.1755-3768.2013.1624.x.. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Presented at: 2015 European Association for Vision and Eye Research Conference (EVER 2013), Nice, France, 18-21 September 2013.

ISSN

1755-375X

eISSN

1755-3768

Language

en

Exports

Logo branding

Keywords

Exports