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Culture on fibrin matrices maintains the colony-forming capacity and osteoblastic differentiation of mesenchymal stem cells

journal contribution
posted on 26.02.2015, 15:28 by Helen Colley, Sally L. McArthur, Alexandra Stolzing, Andrew Scutt
Mesenchymal stem cells (MSC) are multipotent cells capable of differentiating into a number of mesenchymal tissues including bone, cartilage, and tendon. Low numbers in vivo means exponential growth is needed in culture to enable therapeutic applications. MSC can expand rapidly in culture but usually lose their extensive capacity for differentiation that makes them therapeutically attractive. To try and maintain their capacity for differentiation and expansion in vitro, we cultured MSC on fibrin gels of different concentrations to create more physiological growth conditions for the cells. The cells were then re-plated onto tissue culture plastic and analysed. The cells that had been pre-cultured for seven days on fibrin, proliferated and maintained their differential potential to the osteogenic lineage better than tissue culture plastic expanded MSC. A concentration relationship between colony number and fibrin concentration was seen with decreasing numbers as fibrin concentration increased. These data support the concept that substrate signals significantly influence MSC growth and differentiation and that growth on a fibrin matrix could be used to maintain a stem cell phenotype during MSC expansion. © 2012 IOP Publishing Ltd.

Funding

HEC was in receipt of a Departmental Training Allocation studentship from the EPSRC and ASt was funded by the BBSRC. ASc and SLM were HEFCE funded permanent members of staff.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Biomedical Materials (Bristol)

Volume

7

Issue

4

Citation

COLLEY, H. ... et al, 2012. Culture on fibrin matrices maintains the colony-forming capacity and osteoblastic differentiation of mesenchymal stem cells. Biomedical Materials, 7 (4), 045015.

Publisher

© Institute of Physics Publishing

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2012

Notes

This article is closed access.

ISSN

1748-6041

eISSN

1748-605X

Language

en

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Keywords

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