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Degradation of glycated bovine serum albumin in microglial cells

journal contribution
posted on 04.03.2015 by Alexandra Stolzing, Rebecca Widmer, Tobias Jung, Peter Voss, Tilman Grune
Glycated protein products are formed upon binding of sugars to lysine and arginine residues and have been shown to accumulate during aging and in pathologies such as Alzheimer disease and diabetes. Often these glycated proteins are transformed into advanced glycation end products (AGEs) by a series of intramolecular rearrangements. In the study presented here we tested the ability of microglial cells to degrade BSA-AGE formed by glycation reactions of bovine serum albumin (BSA) with glucose and fructose. Microglial cells are able to degrade BSA-AGEs to a certain degree by proteasomal and lysosomal pathways. However, the proteasome and lysosomal proteases are severely inhibited by cross-linked BSA-AGEs. BSA-AGEs are furthermore able to activate microglial cells. This activation is accompanied by an enhanced degradation of BSA-AGE. Therefore, we conclude that microglial cells are able to degrade glycated proteins, although cross-linked protein-AGEs have an inhibitory effect on proteolytic systems in microglial cells. © 2005 Elsevier Inc. All rights reserved.

Funding

The work of T.G. was supported by DFG, GK 1033 and SFB575

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Free Radical Biology and Medicine

Volume

40

Issue

6

Pages

1017 - 1027

Citation

STOLZING, A. ... et al., 2006. Degradation of glycated bovine serum albumin in microglial cells. Free Radical Biology and Medicine, 40 (6), pp. 1017 - 1027.

Publisher

© Elsevier Inc

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2006

Notes

Closed access

ISSN

0891-5849

Other identifier

S0891584905006659

Language

en

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