File(s) under permanent embargo

Reason: This item is currently closed access.

Detection and quantification of beta-amyloid, pyroglutamyl A beta, and tau in aged canines

journal contribution
posted on 02.10.2015, 08:37 by F. Schmidt, J. Boltze, C. Jager, S. Hofmann, N. Willems, J. Seeger, W. Hartig, Alexandra Stolzing
Canine cognitive dysfunction syndrome is an age-associated disorder that resembles many aspects of human Alzheimer disease. The characterization of canine cognitive dysfunction syndrome has been restricted to selected laboratory dogs and mongrels, thereby limiting our knowledge of potential breed-related and age-related differences. We examined the brains of 24 dogs from various breeds. The frontal cortex, hippocampus, and entorhinal cortex were investigated. Deposits of β-amyloid (Aβ) and tau were analyzed phenotypically and quantified stereologically. In all dogs aged 10 years or older, plaques containing pyroglutamyl Aβ and Aβ8–17 were detected. Within the ventral hippocampus, significantly more pyroglutamyl Aβ plaques were deposited in small and medium dogs than in large dogs. Hyperphosphorylated tau with formation of neurofibrillary tangles was observed in 3 animals aged 13 to 15 years. This study provides the first investigation of pyroglutamyl Aβ in comparison with total Aβ (as shown by Aβ8–17 immunoreactivity) in dogs of different breeds, sizes, and ages. Our results indicate that canine cognitive dysfunction syndrome is relatively common among aged canines, thereby emphasizing the relevance of such populations to translational Alzheimer disease research.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY

Volume

74

Issue

9

Pages

912 - 923 (12)

Citation

SCHMIDT, F. ...et al., 2015. Detection and quantification of beta-amyloid, pyroglutamyl A beta, and tau in aged canines. Journal of Neuropathology and Experimental Neurology, 74 (9), pp. 912 - 923

Publisher

© The American Association of Neuropathologists. Published by Oxford Univ. Press.

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2015

Notes

This is in closed access.

ISSN

0022-3069

Language

en

Exports