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Detection of matrilysin (MMP-7) activity using polypeptide functionalized reduced graphene oxide field-effect transistor sensor.

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posted on 30.03.2016 by Hu Chen, Peng Chen, Jingfeng Huang, Robert Selegard, Mark Platt, Alagappan Palaniappan, Daniel Aili, Alfred I.Y. Tok, Bo Liedberg
A novel approach for rapid and sensitive detection of matrilysin (MMP-7, a biomarker involved in the degradation of vari-ous macromolecules) based on polypeptide (JR2EC) functionalized reduced graphene oxide (rGO) field effect transistor (FET) is reported. MMP-7 specifically digests negatively charged JR2EC immobilized on rGO, thereby modulating the con-ductance of rGO-FET. The proposed assay enabled detection of MMP-7 at clinically relevant concentrations with a limit of detection (LOD) of 10 ng/mL (400 pM), attributed to the significant reduction of the net charge of JR2EC upon digestion by MMP-7. Quantitative detection of MMP-7 in human plasma was further demonstrated with a LOD of 40 ng/mL, illustrating the potential for the proposed methodology for tumor detection and carcinoma diagnostic (e.g. lung cancer and salivary gland cancer). Additionally, excellent specificity of the proposed assay was demonstrated using matrix metallopeptidase 1 (MMP-1), a protease of the same family. With appropriate selection and modification of polypeptides, the proposed assay could be extended for detections of other enzymes with polypeptide digestion capability.

Funding

This research was funded by the Institute for Sports Research, Nanyang Technological University

History

School

  • Science

Department

  • Chemistry

Published in

Analytical chemistry

Citation

CHEN, H. ... et al, 2016. Detection of matrilysin (MMP-7) activity using polypeptide functionalized reduced graphene oxide field-effect transistor sensor. Analytical Chemistry, 88 (6), pp. 2994-2998.

Publisher

© American Chemical Society

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2016-02-18

Notes

This article was published in Analytical Chemistry [© American Chemical Society] and the definitive version is available at: http://dx.doi.org/10.1021/acs.analchem.5b04663

ISSN

0003-2700

eISSN

1520-6882

Language

en

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