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Development of a 3D cell culture system for investigating cell interactions with electrospun fibers

journal contribution
posted on 12.05.2017 by Tao Sun, David Norton, Robert J. McKean, John W. Haycock, Anthony J. Ryan, Sheila MacNeil
There are many variables to be considered in studying how cells interact with 3D scaffolds used in tissue engineering. In this study we investigated the influence of the fiber diameter and interfiber spaces of 3D electrospun fiber scaffolds on the behavior of human dermal fibroblasts. Fibers of two dissimilar model materials, polystyrene and poly-L-lactic acid, with a broad range of diameters were constructed in a specifically developed 3D cell culture system. When fibroblasts were introduced to freestanding fibers, and encouraged to “walk the plank,” a minimum fiber diameter of 10 µm was observed for cell adhesion and migration, irrespective of fiber material chemistry. A distance between fibers of up to 200 µm was also observed to be the maximum gap that could be bridged by cell aggregates—a behavior not seen in conventional 2D culture. This approach has identified some basic micro-architectural parameters for electrospun scaffold design and some key differences in fibroblast growth in 3D. We suggest the findings will be of value for optimizing the integration of cells in these scaffolds for skin tissue engineering.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Biotechnology and Bioengineering

Volume

97

Issue

5

Pages

1318 - 1328

Citation

SUN, T. ... et al, 2007. Development of a 3D cell culture system for investigating cell interactions with electrospun fibers. Biotechnology and Bioengineering, 97 (5), pp.1318-1328

Publisher

© Wiley Periodicals, Inc.

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2007

Notes

This paper is closed access.

ISSN

0006-3592

eISSN

1097-0290

Language

en

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