File(s) under embargo

Reason: Publisher requirement

184

days

9

hours

until file(s) become available

Direct detection of small molecules using a nano-molecular imprinted polymer receptor and a quartz crystal resonator driven at a fixed frequency and amplitude

journal contribution
posted on 24.04.2020 by Arnab Guha, Omar Sheej Ahmad, Antonio Guerreiro, Kal Karim, Niklas Sandström, Victor P Ostanin, Wouter van der Wijngaart, Sergey A Piletsky, Sourav Ghosh

Small molecule detection is of wide interest in clinical and industrial applications. However, its accessibility is still limited as miniaturisation and system integration is challenged in reliability, costs and complexity. Here we combined a 14.3 MHz quartz crystal resonator (QCR), actuated and analysed using a fixed frequency drive (FFD) method, with a nanomolecular imprinted polymer for label-free, realtime detection of N-hexanoyl-L-homoserine lactone (199 Da), a gram-negative bacterial infection biomarker. The lowest concentration detected (1 µM) without any optimisation was comparable with that of a BIAcore SPR system, an expensive laboratory gold standard, with significant enhancement in sensitivity and specificity beyond the state-of-the-art QCR. The analytical formula-based FFD method can potentially allow a multiplexed “QCR-on-chip” technology, bringing a paradigm shift in speed, accessibility and affordability of small molecule detection.

Funding

EU projects RAPP-ID (FP7-JTI 115153)

Norosensor (FP7-NMP 604244)

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Biosensors and Bioelectronics

Volume

158

Pages

112176

Publisher

Elsevier BV

Version

AM (Accepted Manuscript)

Rights holder

© Elsevier

Publisher statement

This paper was accepted for publication in the journal Biosensors and Bioelectronics and the definitive published version is available at https://doi.org/10.1016/j.bios.2020.112176

Acceptance date

23/03/2020

Publication date

2020-03-27

Copyright date

2020

ISSN

0956-5663

Language

en

Depositor

Dr Arnab Guha Deposit date: 23 April 2020

Article number

112176

Exports