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Emergence of tunable resistive pulse sensing as a biosensor

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journal contribution
posted on 27.02.2015 by Emma Blundell, Laura J. Mayne, Emily R. Billinge, Mark Platt
The article is written as a guide and tutorial that focuses on the use of Tunable Resistive Pulse Sensing, TRPS, as a platform for the detection of biological analytes. Within the field of biosensors there is a continuous emergence of new technologies or adaptations to platforms that push the limits of detection or expand dynamic ranges. TRPS is both unique and powerful in its ability to detect a wide range of biological analytes; including metabolites, proteins, cellular vesicles, viruses and whole cells. Each analyte can be analysed on the same platform without modification by changing the pore size, and is simple enough to follow to allow users from a range of backgrounds to start developing their own assays. The instrument can provide information regarding analyte concentration, size, and charge. Here we hope to give an overview of where this technology is being used and provide some guidance to new users, in the hope it will inspire and enable future experiments.

Funding

The work was supported by the European Commission for Research (PCIG11-GA-2012-321836 Nano4Bio) and Loughborough University Chemistry Department (Start-up fund). The research was supported by the National Institute for Health Research (NIHR) Diet, Lifestyle & Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University. L. Mayne is supported by EPSRC [grant number EP/L014041/1]. Emma Blundell is sponsored by Izon Science Ltd.

History

School

  • Science

Department

  • Chemistry

Published in

Analytical Methods: advancing methods and applications

Volume

4

Issue

4

Pages

1 - ?

Citation

Blundell, E.L.C.J. ... et al, 2015. Emergence of tunable resistive pulse sensing as a biosensor. Analytical Methods, 7(17), pp.7055-7066.

Publisher

Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2015

Notes

This is an Open Access article licensed under a Creative Commons Attribution 3.0 Unported Licence.

ISSN

1759-9679

Language

en

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