Emergence of tunable resistive pulse sensing as a biosensor
journal contributionposted on 27.02.2015 by Emma Blundell, Laura J. Mayne, Emily R. Billinge, Mark Platt
Any type of content formally published in an academic journal, usually following a peer-review process.
The article is written as a guide and tutorial that focuses on the use of Tunable Resistive Pulse Sensing, TRPS, as a platform for the detection of biological analytes. Within the field of biosensors there is a continuous emergence of new technologies or adaptations to platforms that push the limits of detection or expand dynamic ranges. TRPS is both unique and powerful in its ability to detect a wide range of biological analytes; including metabolites, proteins, cellular vesicles, viruses and whole cells. Each analyte can be analysed on the same platform without modification by changing the pore size, and is simple enough to follow to allow users from a range of backgrounds to start developing their own assays. The instrument can provide information regarding analyte concentration, size, and charge. Here we hope to give an overview of where this technology is being used and provide some guidance to new users, in the hope it will inspire and enable future experiments.
The work was supported by the European Commission for Research (PCIG11-GA-2012-321836 Nano4Bio) and Loughborough University Chemistry Department (Start-up fund). The research was supported by the National Institute for Health Research (NIHR) Diet, Lifestyle & Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University. L. Mayne is supported by EPSRC [grant number EP/L014041/1]. Emma Blundell is sponsored by Izon Science Ltd.