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Expansion of human mesenchymal stem cells on microcarriers

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journal contribution
posted on 06.12.2011 by Christopher Hewitt, Kenneth Lee, Alvin W. Nienow, Rob Thomas, M. Smith, Colin R. Thomas
The effects on human mesenchymal stem cell growth of choosing either of two spinner flask impeller geometries, two microcarrier concentrations and two cell concentrations (seeding densities) were investigated. Cytodex 3 microcarriers were not damaged when held at the minimum speed, NJS, for their suspension, using either impeller, nor was there any observable damage to the cells. The maximum cell density was achieved after 8–10 days of culture with up to a 20-fold expansion in terms of cells per microcarrier. An increase in microcarrier concentration or seeding density generally had a deleterious or neutral effect, as previously observed for human fibroblast cultures. The choice of impeller was significant, as was incorporation of a 1 day delay before agitation to allow initial attachment of cells. The best conditions for cell expansion on the microcarriers in the flasks were 3,000 microcarriers ml−1 (ca. 1 g dry weight l−1), a seeding density of 5 cells per microcarrier with a 1 day delay before agitation began at NJS (30 rpm), using a horizontally suspended flea impeller with an added vertical paddle. These findings were interpreted using Kolmogorov’s theory of isotropic turbulence.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Biotechnology Letters

Volume

33

Issue

11

Pages

2325 - 2335

Citation

HEWITT, C.J. ... et al, 2011. Expansion of human mesenchymal stem cells on microcarriers. Biotechnology Letters, 33 (11), pp. 2325-2335.

Publisher

© Springer Verlag

Version

AM (Accepted Manuscript)

Publication date

2011

Notes

This article was published in the journal, Biotechnology Letters [© Springer Verlag]. The definitive version is available at: http://www.springerlink.com/content/57t4lj6553956p07/

ISSN

0141-5492

Language

en

Exports