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Glucose diffusivity in cell culture medium

journal contribution
posted on 03.02.2015, 14:40 by Hazwani Suhaimi, Shuai Wang, Diganta Das
The diaphragm cell method (DCM) was applied to determine the self-diffusion of glucose in cell culture media (CCM) and water (reference fluid). For this purpose, a diffusion cell based on the DCM principle was constructed and the cell was calibrated with ethanol prior to carrying out the diffusion experiments. The results show that the diffusion coefficients of glucose in CCM are significantly smaller as compared to those for water which are due to the larger dynamic viscosity of CCM. This may also account for the presence of extra components and difference in fluid properties of CCM. The diffusivity values obtained from the present study are compared to values estimated from Stokes-Einstein equation and it appears that the agreement is fairly good.

Funding

The authors gratefully acknowledge a PhD studentship to Hazwani Suhaimi by Brunei Government which made this work possible. Department of Chemical Engineering, Loughborough University is acknowledged for its financial support to Shuai Wang’s PhD work.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Chemical Engineering Journal

Volume

0

Issue

0

Pages

0 - ?

Citation

SUHAIMI, H., WANG, S. and DAS, D.S., 2015. Glucose diffusivity in cell culture medium. Chemical Engineering Journal, 269, pp.323-327.

Publisher

© Elsevier

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2015

Notes

Closed access. This is the author’s version of a work that was accepted for publication in Chemical Engineering Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version will be published at: DOI: 10.1016/j.cej.2015.01.130

ISSN

1385-8947

Language

en

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