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Identification of a glass substrate to study cells using fourier transform infrared spectroscopy: are we closer to spectral pathology?

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journal contribution
posted on 06.03.2020 by Abigail V Rutter, Jamie Crees, Helen Wright, Marko Raseta, Daniel G van Pittius, Paul Roach, Josep Sulé-Suso
The rising incidence of cancer worldwide is causing an increase in the workload in pathology departments. This, coupled with advanced analysis methodologies, supports a developing need for techniques that could identify the presence of cancer cells in cytology and tissue samples in an objective, fast, and automated way. Fourier transform infrared (FT-IR) microspectroscopy can identify cancer cells in such samples objectively. Thus, it has the potential to become another tool to help pathologists in their daily work. However, one of the main drawbacks is the use of glass substrates by pathologists. Glass absorbs IR radiation, removing important mid-IR spectral data in the fingerprint region (1800 cm−1 to 900 cm−1). In this work, we hypothesized that, using glass coverslips of differing compositions, some regions within the fingerprint area could still be analyzed. We studied three different types of cells (peripheral blood mononuclear cells, a leukemia cell line, and a lung cancer cell line) and lymph node tissue placed on four different types of glass coverslips. The data presented here show that depending of the type of glass substrate used, information within the fingerprint region down to 1350 cm−1 can be obtained. Furthermore, using principal component analysis, separation between the different cell lines was possible using both the lipid region and the fingerprint region between 1800 cm−1 and 1350 cm−1. This work represents a further step towards the application of FT-IR microspectroscopy in histopathology departments.

History

School

  • Science

Department

  • Chemistry

Published in

Applied Spectroscopy

Volume

74

Issue

2

Pages

178 - 186

Publisher

SAGE Publications

Version

AM (Accepted Manuscript)

Rights holder

© The Authors

Publisher statement

This paper was accepted for publication in the journal Applied Spectroscopy and the definitive published version is available at https://doi.org/10.1177/0003702819875828. Users who receive access to an article through a repository are reminded that the article is protected by copyright and reuse is restricted to non-commercial and no derivative uses. Users may also download and save a local copy of an article accessed in an institutional repository for the user's personal reference.

Acceptance date

19/08/2019

Publication date

2019-09-13

Copyright date

2019

ISSN

0003-7028

eISSN

1943-3530

Language

en

Depositor

Dr Paul Roach. Deposit date: 5 March 2020

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