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Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model

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journal contribution
posted on 05.10.2017 by Mark Ahearne, Sammy Wilson, Kuo-Kang Liu, Saaeha Rauz, Alicia J. El Haj, Ying Yang
The effect of different collagen and cell concentrations on the mechanical and remodeling behaviors of corneal stroma wound healing models consisting of collagen hydrogels seeded with human corneal fibroblasts during a 25 day culture period were examined. Human corneal fibroblasts were seeded at 1 × 105, 3 × 105 or 5 × 105 cells per hydrogel, and collagen concentrations of 2.5 mg/ml, 3.5 mg/ml or 4.5 mg/ml were examined. Two non-destructive techniques, spherical indentation and optical coherence tomography, were used to measure the elastic modulus and dimensional changes respectively at several time-points over the culture period. The elastic modulus of the hydrogels increased continuously over 25 days. Hydrogels with higher initial cell seeding densities and lower initial collagen concentrations were found to increase in elastic modulus faster and possessed a higher elastic modulus by the end of the culture period when compared to the other hydrogels. A mathematical equation was applied to accurately fit the change in elastic modulus over time. This study demonstrates a robust in vitro technique able to monitor the effect of different parameters on the cell–matrix mechanical relationship in a corneal stroma model during prolonged culture periods and enhances our understanding on corneal wound healing processes.

Funding

This research was funded by BBSRC (BB/F002866/1).

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Experimental Eye Research

Volume

91

Issue

5

Pages

584 - 591

Citation

AHEARNE, M. ... et al., 2010. Influence of cell and collagen concentration on the cell–matrix mechanical relationship in a corneal stroma wound healing model. Experimental Eye Research, 91 (5), pp.584-591.

Publisher

© Elsevier

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2010

Notes

This paper was submitted for publication in the journal Experimental Eye Research and the definitive published version is available at https://doi.org/10.1016/j.exer.2010.07.013

ISSN

0014-4835

Language

en

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