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Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask

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journal contribution
posted on 29.11.2012 by Andrew Want, Alvin W. Nienow, Christopher Hewitt, Karen Coopman
Human pluripotent stem cells will likely be a significant part of the regenerative medicine-driven healthcare revolution. In order to realize this potential, culture processes must be standardized, scalable and able to produce clinically relevant cell numbers, whilst maintaining critical biological functionality. This review comprises a broad overview of important bioprocess considerations, referencing the development of biopharmaceutical processes in an effort to learn from current best practice in the field. Particular focus is given to the recent efforts to grow human pluripotent stem cells in microcarrier or aggregate suspension culture, which would allow geometric expansion of productive capacity were it to be fully realized. The potential of these approaches is compared with automation of traditional T-flask culture, which may provide a cost-effective platform for low-dose, low-incidence conditions or autologous therapies. This represents the first step in defining the full extent of the challenges facing bioprocess engineers in the exploitation of large-scale human pluripotent stem cell manufacture.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Citation

WANT, A. ... et al., 2012. Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask. Regenerative Medicine, 7 (1), pp.71-84.

Publisher

© Future Medicine Ltd

Version

SMUR (Submitted Manuscript Under Review)

Publication date

2012

Notes

This is the submitted for publication version of an article which was published in the journal, Regenerative Medicine [© Future Medicine Ltd] and the definitive version is available at: http://dx.doi.org/10.2217/rme.11.101

ISSN

1746-0751

Language

en

Exports