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Liquid crystalline ordered collagen substrates for applications in tissue engineering

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journal contribution
posted on 25.01.2017, 10:01 by Joshua C. Price, Paul Roach, Alicia J. El Haj
This report describes methods for fabricating substrates with anisotropic order from a single solution of high concentration collagen. By exploiting the intrinsic property of collagen to behave as a cholesteric liquid crystal, we demonstrate first the production of dense collagen films containing anisotropic fibers by simple dialysis and polymerization in ammonia vapor. We then utilized shear driven alignment of collagen using viscous extrusion to produce aligned collagen fibers. Next we describe an evaporation technique to observe crystalline growth into the collagen, which serves to template the substrate prior to fibrillogenesis. The ordered substrates supported osteogenic differentiation of hMSCs and also oriented growth of hMSCs. We also demonstrate using Raman spectroscopy that the local protein concentration in the substrates influenced the molecular orientation of collagen, Finally, we compare the resultant textures in the substrates with section of native cornea and tendon using polarized light microscopy, which showed remarkable similarities in terms of both anisotropy and second order chiral structure. These rapid, cost-effective methods could potentially serve a range of different applications in tissue engineering.

Funding

This research was funded by EPSRC DTC in Regenerative medicine, Keele University. Grant funding number: EP/F500491/1

History

School

  • Science

Department

  • Physics

Published in

ACS Biomaterials Science & Engineering

Volume

2

Issue

4

Pages

625 - 633

Citation

PRICE, J.C., ROACH, P. and EL HAJ, A.J., 2016. Liquid crystalline ordered collagen substrates for applications in tissue engineering. ACS Biomaterials Science & Engineering, 2(4), pp. 625-633.

Publisher

© American Chemical Society

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

03/03/2016

Publication date

2016-03-03

Copyright date

2016

Notes

This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsbiomaterials.6b00030

ISSN

2373-9878

eISSN

2373-9878

Language

en

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