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Mechanistic insights of cells in porous scaffolds via integrated culture technologies

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journal contribution
posted on 11.01.2018 by Chris Gabbott, Tao Sun
This research aimed to combine 3 cell and tissue culture technologies to obtain mechanistic insights of cells in porous scaffolds. When cultivated on 2D (2-dimensional) surfaces, HDFs (human dermal fibroblasts) behaved individually and had no strict requirement on seeding density for proliferation; while HaCat cells relied heavily on initial densities for proliferation and colony formation, which was facilitated when co-cultured with HDFs. Experiments using a 3D CCIS (3-dimensional cell culture and imaging system) indicated that HDFs colonised open pores of varying sizes (125-420 μm) on modular substrates via bridge structures; while HaCat cells formed aperture structures and only colonised small pores (125 μm). When co-cultured, HDFs not only facilitated HaCat attachment on the substrates, but also coordinated with HaCat cells to colonise open pores of varying sizes via bridge and aperture structures. Based on these observations, a 2-stage strategy for the culture of HDFs and HaCat cells on porous scaffolds was proposed and applied successfully on a cellulosic scaffold. This research demonstrated that cell colonisation in scaffolds was dependent on multiple factors; while the integrated 2D&3D culture technologies and the 3D CCIS was an effective and efficient approach to obtain mechanistic insights of their influences on tissue regeneration.

Funding

This study was funded by the Engineering and Physical Sciences Research Council (EPSRC; UK) (EP/L015072/1).

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Journal of Life Sciences

Volume

11

Issue

4

Pages

163-175

Citation

GABBOTT, C. and SUN, T., 2017. Mechanistic insights of cells in porous scaffolds via integrated culture technologies. Journal of Life Sciences, 11 (4), pp. 163-175.

Publisher

David Publishing Company

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by-nc/4.0/

Publication date

2017-04-28

Notes

This is an Open Access Article. It is published by the David Publishing Company under the Creative Commons Attribution-NonCommercial 4.0 International Licence (CC BY-NC). Full details of this licence are available at: http://creativecommons.org/licenses/by-nc/4.0/

ISSN

1934-7391

eISSN

1934-7405

Language

en

Exports