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Microparticle responses to aerobic exercise and meal consumption in healthy men

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journal contribution
posted on 02.04.2019 by Patrick J. Highton, Fernanda R. Goltz, Naomi Martin, David Stensel, Alice Thackray, Nicolette Bishop
PURPOSE: Microparticles (MPs) are shed extracellular vesicles that express the pro-thrombotic tissue factor (TF). Aerobic exercise may reduce MP count and TF expression. This study investigated the impact of acute running or rest followed by standardised meal consumption on MP phenotypes and TF expression. METHODS: 15 males (age: 22.9 ± 3.3 years; body mass: 81.9 ± 11.4 kg; V[Combining Dot Above]O2 max 54.9 ± 6.5 mL·kg·min; mean ± SD) completed 1h of running (70% V[Combining Dot Above]O2max) or rest at 9am, and consumed a standardised meal (1170 kcal, 43% CHO, 17% PRO, 40% fat) at 10:45am. Venous blood samples were taken at 9am, 10am and 11:30am. MP concentration, diameter, phenotypes and TF-expression were assessed using nanoparticle tracking analysis (NTA) and flow cytometry. RESULTS: NTA identified no changes in MP concentration or diameter in response to time or trial. Flow cytometry revealed total MP count increased from 9am to 10am (1.62 ± 2.28 to 1.74 ± 2.61 x10/L, p = .016, effect size (η) = .105), but was unaffected by trial. TF platelet-derived MP % reduced from 9am to 10am (44.0 ± 21.2 to 21.5 ± 9.3%, p = .001, η = .582) after exercise only (control: 36.8 ± 18.2 to 34.9 ± 11.9%, p = .972). TF neutrophil-derived MP % reduced from 9am to 11:30am (42.3 ± 17.2 to 25.1 ± 14.9%, p = 0.048, η = .801) in the exercise trial only (control: 28.5 ± 15.7 to 32.2 ± 9.6%, p = .508). CONCLUSION: Running induced a significant reduction in %TF platelet and neutrophil MP, suggesting a transient reduction in cardiovascular risk via reduced TF-stimulated thrombosis. This requires further investigation over longer time periods in cardiovascular disease populations.

Funding

This research was supported by the NIHR Leicester Biomedical Research Centre. Current affiliation for Patrick Highton: Collaborations for Leadership in Applied Health and Research Care (CLAHRC) within the Diabetes Research Centre, University of Leicester, Leicester, United Kingdom.

History

School

  • Sport, Exercise and Health Sciences

Published in

Medicine and Science in Sports and Exercise

Volume

51

Issue

9

Pages

1935 - 1943

Citation

HIGHTON, P.J. ... et al, 2019. Microparticle responses to aerobic exercise and meal consumption in healthy men. Medicine and Science in Sports and Exercise, 51 (9), pp.1935-1943.

Publisher

Lippincott, Williams & Wilkins

Version

AM (Accepted Manuscript)

Rights holder

© American College of Sports Medicine

Publisher statement

This is a non-final version of an article published in final form in HIGHTON, P.J. ... et al, 2019. Microparticle responses to aerobic exercise and meal consumption in healthy men. Medicine and Science in Sports and Exercise, 51 (9), pp.1935-1943

Acceptance date

15/03/2019

Publication date

2019-09-01

ISSN

0195-9131

eISSN

1530-0315

Language

en

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