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Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

journal contribution
posted on 11.06.2015 by Maria Moffa, Alessandro Polini, Anna G. Sciancalepore, Luana Persano, Elisa Mele, L. Gioia Passione, Giovanni Potente, Dario Pisignano
The objective of this study is elucidating the mechanisms by which the wettability of nanofibrous electrospun mats varies in polymer blends, and highlighting how this can play a pivotal role in enhancing the viability of cultured microvascular endothelial cells (EC). A functional microvascular network is essential for supplying bioengineered tissues with oxygen and nutrients while removing metabolic wastes. An in vitro pre-vascularization strategy consists of seeding EC on scaffolds, which in turn promotes cells infiltration, adhesion and functionality. We use electrospun poly-L-lactic acid (PLLA) and gelatin (Gel) as prototype materials for realizing nanofibrous scaffolds as bioartificial architectures to improve the proliferation and the functionality of human microvascular ECs (HMEC-1). HMEC-1 seeded on electrospun scaffolds adhere, remain viable, proliferate and positively express the endothelial cell marker CD31 particularly on blend PLLA/Gel fibers, which exhibit wettability enhanced with respect to both the constituent polymers, and are therefore especially promising constructs for promoting the formation of functional endothelial tissue. The wettability characteristics of the blend polymer fibrous scaffolds are modeled and discussed. These results can be valuable for the future design of pre-vascularized scaffolds with enhanced wettability properties for functional tissue engineered implants, with ECs able to form in perspective an effectively functioning vasculature upon implantation.

Funding

The authors acknowledge the support of the Italian Ministry of University and Research through the FIRB Contract RBNE08BNL7 (MERIT Program).

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Materials

Published in

SOFT MATTER

Volume

9

Issue

23

Pages

5529 - 5539 (11)

Citation

MOFFA, M. ... et al, 2013. Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability. Soft Matter, 9 (23), pp. 5529 - 5539.

Publisher

© Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2013

Notes

This article is closed access.

ISSN

1744-683X

Language

en

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