REVIEW GE-ICP-MS (Revised).pdf (1.07 MB)

On-line coupling of gel electrophoresis and inductively coupled plasma-mass spectrometry

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journal contribution
posted on 08.01.2016, 09:21 by Syed R. Haider, Barry Sharp, Helen Reid
The on-line coupling of gel electrophoresis with inductively coupled plasma-mass spectrometry (GE-ICP-MS) is a powerful tool for simultaneous separation, detection and quantification of bio-molecules, and has been applied to the determination of phosphorus in DNA, phosphoproteins, and phosphopeptides, gold in nano-particles, iron in metalloproteins, and iodine in aerosols, and cisplatin-oligonucleotide interactions. However, since the first report in 2005, relatively few papers have been published, perhaps reflecting the lack of familiarity with the benefits of this promising methodology. So, here for the first time, we critically review the applications of GE-ICP-MS, and explore the advantages and the limitations of the technique for various applications. Such scrutiny may be useful in not only the development of the technique but also highlighting its potential in proteomics, genomics and metallomics.

History

School

  • Science

Department

  • Chemistry

Published in

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Volume

30

Issue

11

Pages

1793 - 1808 (16)

Citation

HAIDER, S.R., SHARP, B.L. and REID, H.J., 2011. On-line coupling of gel electrophoresis and inductively coupled plasma-mass spectrometry. TrAC-Trends in Analytical Chemistry, 30(11), pp. 1793-1808.

Publisher

© Elsevier

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2011

Notes

This paper was accepted for publication in the journal TrAC-Trends in Analytical Chemistry and the definitive published version is available at http://dx.doi.org/10.1016/j.trac.2011.04.025

ISSN

0165-9936

Language

en

Exports