Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device
journal contributionposted on 22.04.2015 by Ana I. Barbosa, Poonam Gehlot, Kalpita Sidapra, Alexander D. Edwards, Nuno Reis
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We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60. ng/ml with<7% precision in 13. min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08. ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22. min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.
This work was co-funded by Capillary Film Technology Ltd, Loughborough University and EPSRC [grant no. EP/L013983/1].
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