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Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device

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journal contribution
posted on 22.04.2015 by Ana I. Barbosa, Poonam Gehlot, Kalpita Sidapra, Alexander D. Edwards, Nuno Reis
We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60. ng/ml with<7% precision in 13. min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08. ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22. min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.

Funding

This work was co-funded by Capillary Film Technology Ltd, Loughborough University and EPSRC [grant no. EP/L013983/1].

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Biosensors and Bioelectronics

Volume

70

Pages

5 - 14

Citation

BARBOSA, A. ... et al, 2015. Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70, pp.5-14.

Publisher

Elsevier B.V. (© 2015 The Authors)

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Publication date

2015

Notes

This article was published as Open Access by Elsevier B.V. under the CC BY license(http://creativecommons.org/licenses/by/4.0/).

ISSN

0956-5663

eISSN

1873-4235

Language

en

Licence

Exports