File(s) not publicly available

Reason: This item is currently closed access.

Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph

journal contribution
posted on 12.05.2017 by Tao Sun, Yiping Liu, Fengrong Bu, Ge Chen, Meijuan Wen
method is described for the purification of human prothrombin complex concentrate ( PCC) from Nitschmann fraction Ⅲ by membrane radial column ion2exchange chromatography , which allows large sample volumes to be processed at low operation pressure. The Nitschmann fraction Ⅲ(15 g) was mixed with 1 000 mL 0. 06 mol/ L Tris2HCl (pH 7. 5) . The centrifuged supernatant (10 000 r/ min ,15 min ,20 ℃) was applied onto a DEAE ion2exchange liquid chromatographic column (XK216 DEAE fast flow Sepharose and DEAE membrane radical column chromatography) with almost the same excellent separation efficiency. The parameters of sample flow rate , elution flow rate and sample capacity were optimized.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Se pu = Chinese journal of chromatography / Zhongguo hua xue hui

Volume

18

Pages

350 - 353

Citation

SUN, T. ... et al, 2000. Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph. Sepu = Chinese Journal of Chromatography / Zhongguo hua xue hui, 18, pp.350-353

Publisher

Kexue Chubanshe (© Chinese Academic Journal Electronic Publishing House)

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2000

Notes

This paper is closed access.

ISSN

1872-2059

Language

zh

Exports