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Rapid dynamics of cell-shape recovery in response to local deformations

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journal contribution
posted on 10.12.2018 by Kristina Haase, Tyler Shendruk, Andrew E. Pelling
It is vital that cells respond rapidly to mechanical cues within their microenvironment through changes in cell shape and volume, which rely upon the mechanical properties of cells’ highly interconnected cytoskeletal networks and intracellular fluid redistributions. While previous research has largely investigated deformation mechanics, we now focus on the immediate cell-shape recovery response following mechanical perturbation by inducing large, local, and reproducible cellular deformations using AFM. By continuous imaging within the plane of deformation, we characterize the membrane and cortical response of HeLa cells to unloading, and model the recovery via overdamped viscoelastic dynamics. Importantly, the majority (90%) of HeLa cells recover their cell shape in o1 s. Despite actin remodelling on this time scale, we show that cell-shape recovery time is not affected by load duration, nor magnitude for untreated cells. To further explore this rapid recovery response, we expose cells to cytoskeletal destabilizers and osmotic shock conditions, which uncovers the interplay between actin and osmotic pressure. We show that the rapid dynamics of recovery depend crucially on intracellular pressure, and provide strong evidence that cortical actin is the key regulator in the cell-shape recovery processes, in both cancerous and non-cancerous epithelial cells

Funding

AEP was supported by the Canada Research Chairs (CRC) program and a Province of Ontario Early Researcher Award. This work was supported by a Natural Sciences and Engineering Research Council (NSERC) Discovery Grant, an NSERC Discovery Accelerator Supplement, a CRC Operating Grant and the Canadian Foundation for Innovation Leaders Opportunity Fund. We acknowledge funding from ERC Advanced Grant 291234 MiCE and EMBO funding ALTF181-2013 to TNS.

History

School

  • Science

Department

  • Mathematical Sciences

Published in

Soft Matter

Volume

13

Issue

3

Pages

567 - 577

Citation

HAASE, K., SHENDRUK, T.N. and PELLING, A.E., 2016. Rapid dynamics of cell-shape recovery in response to local deformations. Soft Matter, 13(3), pp. 567-577.

Publisher

© Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

30/11/2016

Publication date

2016

Notes

This paper was submitted for publication to the journal Soft Matter and the definitive published version is available at https://doi.org/10.1039/C6SM02560A

ISSN

1744-683X

eISSN

1744-6848

Language

en

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