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Recent developments in the design of rapid response cells for laser ablation-inductively coupled plasma-mass spectrometry and their impact on bioimaging applications

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journal contribution
posted on 12.02.2016 by Stijn J.M. Van Malderen, Amy Managh, Barry L. Sharp, Frank Vanhaecke
This review covers developments in the design of Laser Ablation (LA) cells, the associated transport tubing assembly,and their coupling to Inductively Coupled Plasma- Mass Spectrometry (ICP-MS) instrumentation. Recent ablation cell designs have reduced the pulse response duration for a single laser shot to <10ms, using the criterion of the full peak width at 1% of the height of the maximum signal intensity. The evolution towards these low dispersion systems has been profoundly influenced by our understanding of processes driving the initial dispersion, of the design aspects of the cell and tubing that influence transport-induced dispersion and transport efficiency, and of limitations imposed by the temporal resolution of ICP-MS instruments, all of which are discussed. Rapid response LA-ICP-MS systems greatly benefit throughput and sensitivity, which are key parameters in 2D and 3D imaging at high lateral resolution. The analysis and imaging of biological material has come to the forefront as a key application of LA- ICP-MS. The impact of the technical developments in LA-ICP-MS systems on emerging applications, including multiplexed metal -tagged antibody detection (for immunohistochemistry), nanoparticle and compound hypo-and hyperaccumulation, and (intra-) cellular /histological studies, is also discussed.

Funding

Amy Managh acknowledges financial support through a Loughborough University Enterprise Fellowship (Engineering and Physical Sciences Research Council, Impact Acceleration Account.

History

School

  • Science

Department

  • Chemistry

Published in

Journal of Analytical Atomic Spectrometry

Volume

31

Issue

2

Pages

423 - 439

Citation

VAN MALDEREN, S.J.M. ...ET AL., 2016. Recent developments in the design of rapid response cells for laser ablation-inductively coupled plasma-mass spectrometry and their impact on bioimaging applications. Journal of Analytical Atomic Spectrometry, 31(2), pp. 423-439.

Publisher

© Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 International (CC BY 3.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by/3.0/

Acceptance date

30/11/2015

Publication date

2015-11-30

Copyright date

2016

Notes

This paper was published as Open Access under a Creative Commons Attribution 3.0 Unported Licence.

ISSN

0267-9477

eISSN

1364-5544

Language

en

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