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Scalable 3D printed molds for human tissue engineered skeletal muscle

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posted on 08.03.2019 by Andrew Capel, Rowan Rimington, Jacob Fleming, Darren J. Player, Luke Baker, Mark Turner, Julia Jones, Neil Martin, Richard Ferguson, Vivek Mudera, Mark Lewis
Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25–500 µL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

Funding

The authors would like to acknowledge Loughborough University, EPSRC (grant reference EP/L02067X/1) for funding and support for this work. In addition, this research was funded by the NIHR Leicester Biomedical Research Centre.

History

School

  • Sport, Exercise and Health Sciences

Published in

Frontiers in Bioengineering and Biotechnology

Volume

7

Citation

CAPEL, A.J. ... et al, 2019. Scalable 3D printed molds for human tissue engineered skeletal muscle. Frontiers in Bioengineering and Biotechnology, 7, Article 20.

Publisher

Frontiers Media © The Authors

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

Acceptance date

28/01/2019

Publication date

2019

Notes

This is an Open Access article. It is published by Frontiers Media under the Creative Commons Attribution 4.0 International License (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

eISSN

2296-4185

Language

en

Licence

Exports