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Scale‐down studies for the scale‐up of a recombinant Corynebacterium glutamicum fed‐batch fermentation; loss of homogeneity leads to lower levels of cadaverine production

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journal contribution
posted on 18.10.2019 by Williams Olughu, Alvin Nienow, Christopher Hewitt, Chris Rielly
BACKGROUND
The loss of efficiency and performance of bioprocesses on scale‐up is well known, but not fully understood. This work addresses this problem, by studying the effect of some fermentation gradients (pH, glucose and oxygen) that occur at the larger scale in a bench‐scale two‐compartment reactor (Plug flow reactor (PFR) + Stirred tank reactor (STR)) using the cadaverine‐producing recombinant Corynebacterium glutamicum DM1945 Δact3 Ptuf‐ldcC_OPT. The new scale‐down strategy developed here studied the effect of increasing the magnitude of fermentation gradients by considering not only the average cell residence time in the PFR (τPFR), but also the mean frequency at which the bacterial cells entered the PFR (fm) section of the two‐compartment reactor.

RESULTS
On implementing this strategy the cadaverine production decreased on average by 26 %, 49 % and 59 % when the τPFR was increased from 1 min to 2 min and then 5 min respectively compared to the control fermentation. The CO2 productivity was highest (3.1‐fold that of the control) at a τPFR of 5 min, but no losses were observed in biomass production. However, the population of viable but non‐culturable cells increased as the magnitude of fermentation gradients was increased.

CONCLUSION
This study demonstrated that C. glutamicum DM1945 Δact3 Ptuf‐ldcC_OPT physiological response was a function of the magnitude of fermentation gradients simulated. The adaptations of a bacterial cell within a heterogeneous environment ultimately result in losses in fermentation productivity as observed here.

Funding

SCILS - Systematic consideration of inhomogeneity at the large scale ERA-IB2 framework (EIB.12.057)

BBSRC grant ref BB/L001284/1

EPSRC DTA

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Journal of Chemical Technology & Biotechnology

Volume

95

Issue

3

Pages

675 - 685

Publisher

John Wiley & Sons Ltd on behalf of Society of Chemical Industry

Version

VoR (Version of Record)

Rights holder

© 2019 The Authors

Publisher statement

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Acceptance date

14/10/2019

Publication date

2019-11-21

Copyright date

2020

ISSN

0268-2575

eISSN

1097-4660

Language

en

Depositor

Prof Chris Rielly. Deposit date: 18 October 2019

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