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The influence of acute moderate-to-high intensity aerobic exercise on markers of immune function and microparticles in renal transplant recipients

journal contribution
posted on 28.11.2019 by Patrick Highton, Alice White, Daniel Nixon, Thomas Wilkinson, Jill Neale, Naomi Martin, Nicolette Bishop, Alice Smith
Renal transplant recipients (RTRs) and non-dialysis chronic kidney disease (ND-CKD) patients display elevated circulating microparticle (MP) counts, whilst RTRs display immunosuppression-induced infection susceptibility. The impact of aerobic exercise on circulating immune cells and microparticles is unknown in RTRs. Fifteen RTRs (age 52.8±14.5 years, estimated glomerular filtration rate [eGFR] 51.7±19.8 ml/min/1.73m2 [mean ± SD]), 16 ND-CKD patients (54. ± 6.3 years, eGFR 61.9±21.0 ml/min/1.73m2, acting as a uremic control group), and 16 HCs (52.2±16.2 years, eGFR 85.6±6.1 ml/min/1.73m2) completed 20 minutes of walking at 60-70% VO2 peak. Venous blood samples were taken pre, post, and 1h post-exercise. Leukocytes and MPs were assessed using flow cytometry. Exercise increased classical (p = 0.001) and non-classical (p = 0.002) monocyte subset proportions but decreased the intermediate subset (p < 0.001) in all groups. Exercise also decreased the percentage of platelet-derived MPs that expressed tissue factor (TF+) in all groups (p = 0.01), though no other exercise-dependent effects were observed. The exercise-induced reduction in intermediate monocyte percentage suggests an anti-inflammatory effect, though this requires further investigation. The reduction in the percentage of TF+ platelet-derived MPs suggests reduced pro-thrombotic potential, though further functional assays are required. Exercise did not cause aberrant immune cell activation, suggesting its safety from an immunological standpoint (ISRCTN38935454).

Funding

Stoneygate Trust

Kidney Research UK

History

School

  • Sport, Exercise and Health Sciences

Published in

American Journal of Physiology: Renal Physiology

Volume

318

Issue

1

Pages

F76-F85

Publisher

American Physiological Society

Version

AM (Accepted Manuscript)

Rights holder

© American Journal of Physiology-Renal Physiology

Publisher statement

This paper was accepted for publication in the journal American Journal of Physiology: Renal Physiology and the definitive published version is available at https://doi.org/10.1152/ajprenal.00332.2019.

Acceptance date

14/11/2019

Publication date

2019-11-18

Copyright date

2020

ISSN

1931-857X

eISSN

1522-1466

Language

en

Depositor

Dr Lettie Bishop. Deposit date: 28 November 2019

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