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Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe

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journal contribution
posted on 19.11.2020, 09:02 by Colum Breen, Robert Pal, Mark Elsegood, Simon J Teat, Felipe Iza, Kristian Wende, Benjamin Buckley, Stephen Butler

Peroxynitrite (ONOO) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO in cell biology, improved methods for the selective detection and real-time analysis of ONOO are needed. We present a water-soluble, luminescent europium(III) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe to measure ONOO in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu(III) probe, we monitored the production of ONOO in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment.

Funding

This work was supported by Loughborough University's Centre for doctoral training on Gas Plasma Interactions with Organic Liquids, and a Royal Society Research Grant (SJB). The German Federal Ministry of Education and Research (BMBF), grant no. 03Z22DN12 (KW) supported parts of the work.

History

School

  • Mechanical, Electrical and Manufacturing Engineering
  • Science

Department

  • Chemistry

Published in

Chemical Science

Volume

11

Issue

12

Pages

3164 - 3170

Publisher

Royal Society of Chemistry (RSC)

Version

VoR (Version of Record)

Rights holder

© The Royal Society of Chemistry

Publisher statement

This is an Open Access Article. It is published by the Royal Society of Chemistry under the Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0). Full details of this licence are available at: https://creativecommons.org/licenses/by/3.0/

Acceptance date

14/02/2020

Publication date

2020-02-18

Copyright date

2020

ISSN

2041-6520

eISSN

2041-6539

Language

en

Depositor

Dr Felipe Iza. Deposit date: 16 November 2020

Licence

Exports