Sallstrom_2041731420967294.pdf (1.21 MB)
3D-printable zwitterionic nano-composite hydrogel system for biomedical applications
journal contributionposted on 2020-12-10, 15:38 authored by Nathalie Sallstrom, Andrew CapelAndrew Capel, Mark LewisMark Lewis, Daniel EngstromDaniel Engstrom, Simon MartinSimon Martin
Herein, the cytotoxicity of a novel zwitterionic sulfobetaine hydrogel system with a nano-clay crosslinker has been investigated. We demonstrate that careful selection of the composition of the system (monomer to Laponite content) allows the material to be formed into controlled shapes using an extrusion based additive manufacturing technique with the ability to tune the mechanical properties of the product. Moreover, the printed structures can support their own weight without requiring curing during printing which enables the use of a printing-then-curing approach. Cell culture experiments were conducted to evaluate the neural cytotoxicity of the developed hydrogel system. Cytotoxicity evaluations were conducted on three different conditions; a control condition, an indirect condition (where the culture medium used had been in contact with the hydrogel to investigate leaching) and a direct condition (cells growing directly on the hydrogel). The result showed no significant difference in cell viability between the different conditions and cells were also found to be growing on the hydrogel surface with extended neurites present.
EPSRC Centre for Doctoral Training in Additive Manufacturing and 3D Printing
Engineering and Physical Sciences Research CouncilFind out more...
- Mechanical, Electrical and Manufacturing Engineering
- Sport, Exercise and Health Sciences
Published inJournal of Tissue Engineering
Pages1 - 11
- VoR (Version of Record)
Rights holder© The authors
Publisher statementThis is an Open Access Article. It is published by Sage under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/