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A comprehensive comparison of bovine and porcine decellularized pericardia: new insights for surgical applications

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journal contribution
posted on 25.11.2020, 11:59 by Sabra Zouhair, Eleonora Dal Sasso, Sugat R. Tuladhar, Catia Fidalgo, Luca Vedovelli, Andrea Filippi, Giulia Borile, Andrea Bagno, Massimo Marchesan, Giorgio De Rossi, Dario Gregori, Willem F. Wolkers, Filippo Romanato, Sotiris Korossis, Gino Gerosa, Laura Iop
Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.

Funding

CA.RI.PA.RO. Foundation for the Padua Heart Project

European Union’s Seventh Framework Programme for FP7/2007-2013/ITN-TECAS (agreement: 317512)

Consorzio per la Ricerca Sanitaria (CORIS) (DGR. 1017, 17 July 2018), Veneto Region, Italy for L.I.F.E.L.A.B. Program

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Biomolecules

Volume

10

Issue

3

Publisher

MDPI

Version

VoR (Version of Record)

Rights holder

© The Authors

Publisher statement

This is an Open Access Article. It is published by MDPI under the Creative Commons Attribution 4.0 International Licence (CC BY 4.0). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

Acceptance date

17/02/2020

Publication date

2020-02-28

Copyright date

2020

ISSN

2218-273X

eISSN

2218-273X

Language

en

Depositor

Prof Sotiris Korossis. Deposit date: 24 November 2020

Article number

371

Licence

Exports