A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex
journal contributionposted on 2017-09-27, 10:29 authored by Sarah Hewitt, Jonathan Parris, Romain Mailhot, Stephen ButlerStephen Butler
We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
This work was supported by a Wellcome Trust Seed Award (204500/Z/16/Z) and a Royal Society Research Grant.
Published inChemical Communications
CitationHEWITT, S.H. ... et al, 2017. A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex. Chemical Communications, 53 (94), pp. 12626-12629.
Publisher© Royal Society of Chemistry
- AM (Accepted Manuscript)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis paper was published in the journal Chemical Communications and the definitive published version is available at https://doi.org/10.1039/c7cc05887j.