A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

Hewitt, Sarah; Parris, Jonathan; Mailhot, Romain; Butler, Stephen

Revised Manuscript_Kinase Assay_Final.pdf (485.47 kB)

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

journal contribution

posted on 2017-09-27, 10:29 authored by Sarah Hewitt, Jonathan Parris, Romain Mailhot, Stephen ButlerStephen Butler

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

Funding

This work was supported by a Wellcome Trust Seed Award (204500/Z/16/Z) and a Royal Society Research Grant.

History

School

Science

Department

Chemistry

Published in

Chemical Communications

Citation

HEWITT, S.H. ... et al, 2017. A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex. Chemical Communications, 53 (94), pp. 12626-12629.

Publisher

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

2017-09-22

Publication date

2017

Notes

This paper was published in the journal Chemical Communications and the definitive published version is available at https://doi.org/10.1039/c7cc05887j.