A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex
journal contributionposted on 27.09.2017, 10:29 by Sarah Hewitt, Jonathan Parris, Romain Mailhot, Stephen ButlerStephen Butler
We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
This work was supported by a Wellcome Trust Seed Award (204500/Z/16/Z) and a Royal Society Research Grant.