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A lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood

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journal contribution
posted on 28.11.2014, 11:28 by Ana I. Barbosa, Ana P. Castanheira, Alexander D. Edwards, Nuno Reis
We present a new concept for rapid and fully portable prostate specific antigen (PSA) measurements, termed "lab-in-a-briefcase", which integrates an affordable microfluidic ELISA platform utilising a melt-extruded fluoropolymer microcapillary film (MCF) containing an array of 10 200 μm internal diameter capillaries, a disposable multi-syringe aspirator (MSA), a sample tray pre-loaded with all of the required immunoassay reagents, and a portable film scanner for colorimetric signal digital quantification. Each MSA can perform 10 replicate microfluidic immunoassays on 8 samples, allowing 80 measurements to be made in less than 15 minutes based on semi-automated operation, without the need of additional fluid handling equipment. The assay was optimised for the measurement of a clinically relevant range of PSA of 0.9 to 60.0 ng ml in 15 minutes with CVs on the order of 5% based on intra-assay variability when read using a consumer flatbed film scanner. The PSA assay performance in the MSA remained robust in undiluted or 12 diluted human serum or whole blood, and the matrix effect could simply be overcome by extending sample incubation times. The PSA "lab-in-a-briefcase" is particularly suited to a low-resource health setting, where diagnostic labs and automated immunoassay systems are not accessible, by allowing PSA measurement outside the laboratory using affordable equipment.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Lab on a Chip - Miniaturisation for Chemistry and Biology

Volume

14

Issue

16

Pages

2918 - 2928

Citation

BARBOSA, A.I. ... et al., 2014. A lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood. Lab on a Chip - Miniaturisation for Chemistry and Biology, 14 (16), pp. 2918 - 2928.

Publisher

Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2014

Notes

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

ISSN

1473-0197

eISSN

1473-0189

Language

en

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