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Download fileA microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response
journal contribution
posted on 2017-10-05, 15:54 authored by Sammy WilsonSammy Wilson, Marie Guilbert, Josep Sule-Suso, James Torbet, Pierre Jeannesson, Ganesh D. Sockalingum, Ying YangDuring aging, collagen structure changes, detrimentally affecting tissues' biophysical and biomechanical properties due to an accumulation of advanced glycation end-products (AGEs). In this investigation, we conducted a parallel study of microscopic and macroscopic properties of different-aged collagens from newborn to 2-yr-old rats, to examine the effect of aging on fibrillogenesis, mechanical and contractile properties of reconstituted hydrogels from these collagens seeded with or without fibroblasts. In addition to fibrillogenesis of collagen under the conventional conditions, some fibrillogenesis was conducted alongside a 12-T magnetic field, and gelation rate and AGE content were measured. A nondestructive indentation technique and optical coherence tomography were used to determine the elastic modulus and dimensional changes, respectively. It was revealed that in comparison to younger specimens, older collagens exhibited higher viscosity, faster gelation rates, and a higher AGE-specific fluorescence. Exceptionally, only young collagens formed highly aligned fibrils under magnetic fields. The youngest collagen demonstrated a higher elastic modulus and contraction in comparison to the older collagen. We conclude that aging changes collagen monomer structure, which considerably affects the fibrillogenesis process, the architecture of the resulting collagen fibers and the global network, and the macroscopic properties of the formed constructs.
Funding
Funding from the Engineering and Physical Sciences Research Council (EPSRC; Swindon, UK) Doctoral Training Centre (DTC) in Regenerative Medicine (grant EP/F/500491/1), Alliance bilateral program (Egide and British Council) and the exchange program in EuroMagNET II (Grenoble, France) are gratefully acknowledged. M.G. is a recipient of a fellowship from the Région Champagne-Ardenne, France.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
The FASEB JournalVolume
28Issue
1Pages
14 - 25Citation
WILSON, S. ... et al., 2014. A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response. FASEB Journal, 28 (1), pp.14-25.Publisher
© Federation of American Societies for Experimental BiologyVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2014Notes
This paper was submitted for publication in the journal FASEB Journal and the definitive published version is available at https://doi.org/10.1096/fj.13-227579ISSN
0892-6638eISSN
1530-6860Publisher version
Language
- en